Immobilized glucose oxidase and D-amino acid oxidase: a convenient method for the purification of flavin adenine dinucleotide and its analogs.
نویسندگان
چکیده
The preparation of flavin adenine dinucleotide-aftinity columns employing glucose oxidase and D-amino acid oxidase covalently linked to Sepharose-4B is described. Both immobilized enzymes have very good long-term stabilities, retaining at least half of their original flavin adenine dinucleotide-binding capacity over 11 months. In both cases pure flavin adenine dinucleotide is obtained readily in good yields.
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عنوان ژورنال:
- Analytical biochemistry
دوره 95 1 شماره
صفحات -
تاریخ انتشار 1979